Figure 3.

Northern blot analysis of Physcomitrella RAD51 genes. Poly(A)⁺-selected RNA was prepared from actively growing 7-day-old protonema tissue. The RNA (9 μg per lane) was separated by gel electrophoresis and blotted to a membrane, and the membrane was hybridized under high-stringency conditions with RAD51A (A) and RAD51B (B) gene-specific probes. The approximate sizes of the transcripts as deduced from a stained RNA size marker (0.24–9.5-kb RNA ladder, Life Technologies, Karlsruhe, Germany) are given.