Figure 3.

Immunofluorescence and immunoelectron micrographs of human intestinal epithelium sections stained with anti-MICA mouse serum. (A) Human intestinal epithelium was double-stained with anti-MICA mouse serum and FITC-conjugated anti-mouse Ig (green) and anti-intestinal alkaline phosphatase rabbit serum and Texas Red-conjugated anti-rabbit Ig (red). (B) Immunohistochemical detection of MICA in human intestinal epithelium by staining with anti-MICA mouse serum and HRP-conjugated anti-mouse Ig. (C) The ultrastructural localization of MICA molecules in human intestinal epithelial cells was analyzed by immunoelectron microscope employing anti-MICA mouse serum and HRP-conjugated secondary antibody. (Scale bar = 500 nm.)