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. 2002 Feb 5;99(5):2977–2982. doi: 10.1073/pnas.032668099

Figure 3.

Figure 3

Effect of bacteria-induced MICA expression on NK cell IFN-γ production. Caco-2 cells were either uninfected (Caco-2 control) or infected with SC300 (Caco-2 SC300), a noninvasive S. flexneri strain expressing the adhesin AfaE. Before contact with NKL cells, Caco-2 target cells were fixed in PBS/1% paraformaldehyde. Supernatants were assayed for IFN-γ production in ELISA and given as the mean of triplicate measurements. Blocking experiments of IFN-γ release were carried out with preimmune or MICA immune serum (1:50) added to Caco-2 after a 2-h bacterial infection. For blockade of bacterial adhesion, CD55-specific mAb BRIC216 or an Ig-isotypic control (10 μg/ml) was incubated with targets before bacterial infection. In phosphatidylinositol 3-kinase inhibition experiments, NKL were treated for 1 h with 25 mM wortmannin before contact with 1% paraformaldehyde fixed Caco-2 cells.