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Production of NO from mouse peritoneal macrophages. Thioglycolate-elicited peritoneal macrophages were incubated in vitro with increasing concentrations of SAHA for 1 h and then stimulated with the combination of human TNF-α (10 ng/ml) plus murine IFN-γ (100 units/ml). After 24 h, the supernatants were assayed for NO. Mean ± SE of four separate experiments. *, P < 0.05; **, P < 0.01.
