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. 2005 Sep 8;102(38):13502–13507. doi: 10.1073/pnas.0501902102

Fig. 2.

Fig. 2.

Impaired sprouting of ISVs and elevated apoptotic cells around the DA are seen in DMO embryos. (A) Representative photographs of whole-mount ISH of flk-1 at 28 hpf in St and DMO embryos. High magnification images of head portions indicated by dotted grids in the upper left are shown in the lower left. Normal vasculature in head portion is seen in both St and DMO. High magnification images in the trunks indicated by closed grids in the upper left are shown in the right images. Filled arrowheads indicate ISVs with impaired sprouting in DMO. (B) Representative photographs of whole-mount ISH of fli-1 at 12 hpf and flk-1 at 18 hpf in St and DMO embryos. Normal vasculogenesis occurs in DMO-injected embryos. (C) Representative photographs of whole-mount TUNEL assay at 28 hpf. High magnification images of the trunk indicated by dotted grids in Upper are shown in Lower. Open arrowheads indicate increased apoptotic cells in the area of major trunk vessels in DMO. (D) A sagittal section of DMO embryos stained with TUNEL. Open arrowheads indicate apoptotic cells around the DA, and open arrows indicate naturally occurring apoptosis in the neural tube. NC, notochord. (Scale bar: 50 μm.). (E) Representative photographs of somites examined by whole-mount ISH of myoD at 12 hpf, simple microscopic observation at 18 hpf, hematoxylin/eosin staining of sagittal sections at 72 hpf, and whole-mount ISH of vegf at 24 hpf. Somitogenesis and vegf expression are not impaired in DMO embryos. (Scale bar: 100 μm.).