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. 2005 Sep 12;102(38):13568–13573. doi: 10.1073/pnas.0502815102

Fig. 2.

Fig. 2.

The expression profiles of Vg-ΔREL1-A and Vg-iREL1 transgene and transgenic effects on the endogenous REL1 gene expression. (A) Northern blot analysis showing the Vg promoter-mediated activation of the transgenes after blood feeding. Endogenous REL1 transcripts, REL1-A and REL1-B, were detected in the parental mosquitoes at any stage. The additional bands, which appeared after blood feeding, were detected in the Vg-ΔREL1-A and Vg-iREL1 transgenic mosquitoes, respectively. (B and C) Real-time PCR analysis showing the relative level of endogenous REL1 transcripts in transgenic and parental mosquitoes. After blood feeding, the Vg-ΔREL1-A transgenic mosquitoes showed the overexpression of REL1 transcripts, whereas the Vg-iREL1 transgenic mosquitoes showed the knockdown of both REL1 transcripts, REL1-A (B) and REL1-B (C). PV, previtellogenic.