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. 2005 Sep 7;102(38):13461–13466. doi: 10.1073/pnas.0503689102

Fig. 3.

Fig. 3.

FLIM of APP695 and sorLA interaction in N2A cells. Shown are intensity images of sorLA (A and B) and APP695 (C) immunocytochemistry using donor fluorophore Alexa Fluor 488-conjugated antibodies (Left), pseudocolored FLIM images (Center), and lifetime histograms (Right), indicating shortening of lifetime from blue to orange/red in the absence (A) or presence (B and C) of acceptor fluorophore Cy3. (A) Cells stained for the amino-terminal domain of sorLA with donor fluorophore Alexa Fluor 488 in the absence of acceptor (lifetime of 2,007 ± 12 psec). (B) Cells stained for sorLA with donor fluorophore Alexa Fluor 488 in the presence of acceptor Cy3 on the ectodomain of APP695 (lifetime reduced to 1,365 ± 139 psec). (C) Cells stained for APP with donor Alexa Fluor 488 in the presence of acceptor Cy3 on sorLA (lifetime reduced to 1,481 ± 228 psec).