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. 2005 Sep 6;102(38):13658–13663. doi: 10.1073/pnas.0504167102

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Fig. 2. — RPE65, CRALBP, LRAT, and RDH5 are expressed only in transfected 293-F cells. (A) Immunoblot analysis of whole-cell lysates probed with antisera to, from top, RPE65, CRALBP, RDH5, and LRAT. Lane 1, untransfected 293-F cells; lane 2, 293-F cells transfected with an irrelevant plasmid; lane 3, 293-F cells transfected with pVitro2/RPE65+CRALBP and pVitro3/LRAT+RDH5. (B) Single-channel and merged confocal images of 293-F cells transfected with pVitro2/RPE65+CRALBP and labeled to demonstrate coexpression of RPE65 and CRALBP. Immunolabeling: (a) rabbit antibody to RPE565 (green); (b) mouse monoclonal antibody to CRALBP (red); (c) merged triple-labeled image; (d) merged image of untransfected 293 cells labeled with RPE65 and CRALBP antibodies. DAPI labels cell nuclei (blue) in all images.