Fig. 3.

DJ-1A RNAi leads to ROS accumulation and hypersensitivity to oxidative stress. (A and B) Comparison of survival curves of elav-GAL4/+ flies with elav-GAL4>DJ-1A RNAi flies that are treated with 1% H2O2 (A) or 100 mM 3-AT (B). (C and D) DCFH-DA staining of cultured Da-GAL4/+ (C) and Da-GAL4>DJ-1A RNAi (D) neurons. (Upper) Fluorescent DCFH-DA staining in green. (Lower) Black and white images of the neuronal culture being analyzed. (E) Recombinant DJ-1A protein exhibits detectable in vitro H₂O₂ scavenging activity, whereas the control proteins BSA, GST, and PAR-1 have no such activity.