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. 2002 Feb 26;99(5):3013–3017. doi: 10.1073/pnas.042684699

Figure 2.

Figure 2

In vitro response of PLP40–59-primed BALB/cJ lymph node cells. Proliferation (A) and IFN-γ production (B) of unfractionated draining lymph node cells from mice treated with control Ab (open symbols) or anti-CTLA-4 Ab (filled symbols). Mice were immunized with 200 μg of PLP40–59 emulsified in CFA. Abs were administered i.p. on days 0 and 3, and draining lymph node cells were assayed on day 7 after immunization. In round-well plates, 3 × 105 cells per well were cultured with peptide at the indicated concentrations. Supernatants were collected at 72 h for ELISA, and 1 μCi (1 μCi = 37 kBq) of [3H]thymidine was added per well. Proliferation was assessed 12 h later. Results are representative of five separate experiments.