B cells derived from the 1–82 transgenic mice differentiate to mature
cells. (A) Splenocytes from control,
Ii−/−, and 1–82 heterozygotic (hetero) or
homozygotic (homo) mice were double-stained with anti-B220 and either
anti-IgM, anti-CD23, or anti-IgD. Histograms show the expression of the
various markers on B220-positive cells. (B) Splenocytes
from control, Ii−/−, and 1–82 homozygotic mice
were triple-stained with anti-B220 and anti-IgD, anti-IgM, anti-CD23,
and anti-CD21. Dot plots show the expression of the markers on
B220-positive cells. M, mature B cells; T1, transitional B cells 1; T2,
transitional B cells 2; MZ, marginal zone B cells. The results
presented are representative of five different experiments.