Figure 8.

Boat expression is reduced in the Purkinje cells of SCA1 mice at the early stage. Frozen brain sections, prepared from 3-week-old wild-type (FVB/N) mice (A) or from age-matched SCA1^tg/+ mice (B), were co-immunostained with Calbindin antibody along with ataxin-1, SMRT, or Boat antibody. (C) In situ hybridization for 3-week-old wild-type (FVB/N) and SCA1^tg/+ mice, using antisense or sense RNA probes for Boat. The enlarged images show the regions adjacent to the Purkinje cell layer (PCL). (D) A model depicting the relationship between Boat and ataxin-1 in the Purkinje cells of SCA1 mice. Scale bars: 1000 μM.