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(A) Growth curves of TKO MEFs overexpressing C/EBPβ and/or RasV12. Data are the means±s.e. of triplicate assays. Right panel: Western blot for C/EBPβ expression. (B) Colony assays of the TKO MEFs from panel A. (C) Dominant negative E2F disrupts C/EBPβ-induced arrest. Wild-type or C/EBPβ−/− MEFs were infected with vectors for C/EBPβ and/or dominant negative E2F-1 (E2F-DB) and growth curves were performed. Right panel: Western blot analysis of E2F-DB and C/EBPβ expression.
