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. 2005 Aug 18;24(18):3279–3290. doi: 10.1038/sj.emboj.7600791

Figure 9.

Figure 9

Endogenous KAP1 regulates p53 response to DNA damage. (A) HT1080-GT and HT1080-K2 cells were treated with 10 Gy gamma radiation for 6 h and analyzed by Western blot for the indicated protein markers. (B) HT1080-GT and HT1080-K2 cells were treated with 10 Gy gamma radiation for 2 h before significant p53 accumulation occurs and p53 acetylation was analyzed by IP followed by Western blot. (C) HT1080 cells were treated with gamma radiation and cell survival were analyzed by MTT assay after 60 h. Knockdown of KAP1 increased p53 target gene induction after radiation and correlated with increased apoptosis. (D) HT1080 cells were treated with 10 Gy gamma radiation and cleavage of the apoptotic substrate PARP as indicated by the generation of the 87 kDa fragment was analyzed by Western blot after 48 h.