Table 2.
SPR equilibrium constants between Mud1UBA and polyubiquitin
| Ligand | Analyte | Kd (μM) | na |
|---|---|---|---|
| Biotin-Mud1UBA WT | Ubiquitin | 390±50 | 1 |
| Biotin-Mud1UBA WT | N-C-Ub2 | 140±15 | 0.96±0.03 |
| Biotin-Mud1UBA WT | K48-Ub2 | 3.1±0.2 | 0.96±0.02 |
| Biotin-Mud1UBA WT | K48-Ub3 | 2.0±0.2 | 0.88±0.02 |
| Biotin-Mud1UBA WT | K48-Ub4 | 1.1±0.4 | 0.61±0.04 |
| Biotin-Mud1UBA F330A | Ubiquitin | 470±70 | 1 |
| Biotin-Mud1UBA F330A | N-C-Ub2 | 200±50 | 0.95±0.04 |
| Biotin-Mud1UBA F330A | K48-Ub2 | 65±15 | 0.97±0.03 |
| Biotin-Mud1UBA F330A | K48-Ub3 | 45±12 | 0.89±0.03 |
| Biotin-Mud1UBA F330A | K48-Ub4 | 38±8 | 0.82±0.04 |
| Amine-coupled Mud1 | K48-Ub2 | 3.7±0.2 | 0.98±0.02 |
| Amine-coupled Mud1 | K48-Ub4 | 0.06±0.02 | 0.96±0.03 |
| Amine-coupled K48-Ub2 | Mud1UBA WT | 6.9±0.3 | 1 |
| Amine-coupled K48-Ub4 | Mud1UBA WT | 6.0±0.3 | 1 |
| Amine-coupled K48-Ub2 | Mud1UBA F330A | 160±12 | 1 |
| Amine-coupled K48-Ub4 | Mud1UBA F330A | 89±8 | 1 |
| aHill coefficient. |