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. 2005;2005(3):232–237. doi: 10.1155/JBB.2005.232

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Hindawi Publishing Corporation

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Flow cytometric analysis and isolation of EGFP-labeled cranial NCC. Cell suspensions were prepared from the tissue derived from the cranial region, rostral to the second branchial arch, of 9.5-dpc EGFP-labeled embryos. Cells were subjected to fluorescence-activated cell sorting as delineated in the “materials and methods.” Representative histogram (a) shows total population of gated cells. Representative post-sorting histogram (b) indicates EGFP-positive neural crest cells in sector R12. The EGFP-labeled cell population in R12 routinely constitutes approximately 30% of the total gated cells.

Flow cytometric analysis and isolation of EGFP-labeled cranial NCC. Cell suspensions were prepared from the tissue derived from the cranial region, rostral to the second branchial arch, of 9.5-dpc EGFP-labeled embryos. Cells were subjected to fluorescence-activated cell sorting as delineated in the “materials and methods.” Representative histogram (a) shows total population of gated cells. Representative post-sorting histogram (b) indicates EGFP-positive neural crest cells in sector R12. The EGFP-labeled cell population in R12 routinely constitutes approximately 30% of the total gated cells.