DNase I HS sites in AML1 (A–C) and
ETO (D–F).
(A) EcoRI digestion and
hybridization with A12. A 3.9-kb DNA fragment was detected, indicating
a DNase I HS site colocalizing with topo II site C in intron 5.
(B) EcoRI digestion and hybridization
with probe A20. Three DNA fragments of 1.5, 1.6, and 1.8 kb were
detected, indicating three DNase I HS sites colocalizing with topo II
sites D, E, and F. (C) BamHI digestion
and hybridization with A22. A 2.7-kb DNA fragment was identified,
indicating one DNase I HS site colocalizing with topo II site I.
(D) BamHI digestion and hybridization
with probe E1. Two DNA fragments of 1.9 kb and 5.7 kb were detected,
thus two DNase I HS sites colocalize with topo II sites a and b,
respectively. (E) EcoRI digestion and
hybridization with probe E2. A 3.5-kb DNA fragment was identified,
confirming the DNase I HS site that colocalizes with topo II site a.
(F) BamHI digestion and hybridization
with E6. A 4.2-kb DNA fragment was detected, confirming the DNase I HS
site colocalizing with topo II site b.