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. 2002 Feb 26;99(5):3070–3075. doi: 10.1073/pnas.042702899

Figure 4.

Figure 4

DNase I HS sites in AML1 (A–C) and ETO (D–F). (A) EcoRI digestion and hybridization with A12. A 3.9-kb DNA fragment was detected, indicating a DNase I HS site colocalizing with topo II site C in intron 5. (B) EcoRI digestion and hybridization with probe A20. Three DNA fragments of 1.5, 1.6, and 1.8 kb were detected, indicating three DNase I HS sites colocalizing with topo II sites D, E, and F. (C) BamHI digestion and hybridization with A22. A 2.7-kb DNA fragment was identified, indicating one DNase I HS site colocalizing with topo II site I. (D) BamHI digestion and hybridization with probe E1. Two DNA fragments of 1.9 kb and 5.7 kb were detected, thus two DNase I HS sites colocalize with topo II sites a and b, respectively. (E) EcoRI digestion and hybridization with probe E2. A 3.5-kb DNA fragment was identified, confirming the DNase I HS site that colocalizes with topo II site a. (F) BamHI digestion and hybridization with E6. A 4.2-kb DNA fragment was detected, confirming the DNase I HS site colocalizing with topo II site b.