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. Author manuscript; available in PMC: 2005 Sep 21.
Published in final edited form as: J Biol Chem. 2005 Mar 17;280(19):18728–18735. doi: 10.1074/jbc.M501265200

Fig. 7. Temperature dependence of MTSET labeling.

Fig. 7

HRPE cells transiently expressing MTSET-sensitive cysteine mutants were preincubated in sodium buffer (Na) with or without 10 mm succinate (S) and with or without MTSET. The MTSET concentrations were 10 μm (T484C, L485C, I489C, and M493C) and 1 mm (T483C and L487C). The preincubations were done at room temperature (RT) or on ice for 10 min. After the pretreatment, cells were washed and allowed to come to room temperature, and then uptakes of [3H]succinate were measured as described previously. Data shown are means ± range or S.E. (n = 2–6 experiments). *, significant differences (p < 0.05) between groups incubated at room temperature and on ice.