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. Author manuscript; available in PMC: 2006 Jul 1.
Published in final edited form as: J Nutr. 2005 Jul;135(7):1659–1666. doi: 10.1093/jn/135.7.1659

Fig. 2.

Fig. 2

The relative abundance of proteins from high-throughput immunoblot analysis was confirmed by using one-dimensional gel electrophoresis. HepG2 cells were cultured in media containing deficient (0.025 nmol/L), physiological (0.25 nmol/L), and pharmacological (10 nmol/L) concentrations of biotin for 10 d. High-throughput immunoblot analysis suggested that the abundance of the following proteins increased in response to biotin deficiency: SAM68 (SwissProt ID: Q07666); GRB2 (SwissProt ID: P29354); A-RAF (SwissProt ID: P04627). Here, these findings were validated by Western blot analysis using a one-dimensional gel electrophoresis system.