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. 2002 Feb 26;99(5):3123–3128. doi: 10.1073/pnas.052712199

Figure 7.

Figure 7

PCR detection of the transgene in peripheral blood of the dog. A fragment of the bicistronic γc-IRES-MDR1 cDNA was amplified from 200 ng of DNA with primers specific for the γc (sense primer) and IRES (antisense primer). As a control, a fragment from the canine corticotrophin-releasing hormone gene was amplified under identical conditions. Shown are analyses from blood at the following time points: 17 months post-BMT (lane 1), 25 months (lane 2), 27 months (lane 3), 28 months (lane 4), and 43 months post-BMT (lane 5). As a negative control, blood from a normal, untreated animal is shown in lane 6.