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. 2002 Feb 19;99(5):3129–3134. doi: 10.1073/pnas.052694299

Figure 4.

Figure 4

LuxO regulation of hapR expression and HapR regulation of CT production. (A) Wild-type and luxO mutants carrying a chromosomal hapR-lacZ reporter fusion were grown in AKI medium at 37°C. Samples were withdrawn at the specified ODs, and β-galactosidase activity was assayed. ●, wild type (wt); ▴, luxO mutant. (B) Wild-type V. cholerae containing the vector pMal-c2x or carrying pJZ146 (pMal-c2X containing hapR under IPTG control) were grown as described in AKI medium, and IPTG (50 μM final concentration) was added at the indicated time points. All of the samples were assayed for CT production after a total of 10 h at 37°C. The data are presented as the percentage of CT production in the V. cholerae strain carrying pJZ146 compared with the strain carrying only the vector pMal-c2x.