Table 2.
Differential gene expression in the luxO mutant in AKI medium
| ORF | ID | Fold activation |
|---|---|---|
| Pathogenesis | ||
| ToxR-activated gene A protein, TagA | VC0820 | −3.6 |
| TagD protein | VC0824 | −13.4 |
| Toxin coregulated pilus biosynthesis protein, TcpI | VC0825 | −7.5 |
| Toxin coregulated pilus biosynthesis protein, TcpP | VC0826 | −16.6 |
| Toxin coregulated pilus biosynthesis protein, TcpH | VC0827 | −10.2 |
| Toxin coregulated pilin, TcpA | VC0828 | −41.7 |
| Toxin coregulated pilus biosynthesis protein, TcpB | VC0829 | −29.3 |
| Toxin coregulated pilus biosynthesis protein, TcpQ | VC0830 | −22.1 |
| Toxin coregulated pilus biosynthesis protein, TcpC | VC0831 | −35.9 |
| Toxin coregulated pilus biosynthesis protein, TcpR | VC0832 | −18.8 |
| Toxin coregulated pilus biosynthesis protein, TcpD | VC0833 | −37.8 |
| Toxin coregulated pilus biosynthesis protein, TcpS | VC0834 | −53.9 |
| Toxin coregulated pilus biosynthesis protein, TcpT | VC0835 | −29.7 |
| Toxin coregulated pilus biosynthesis protein, TcpE | VC0836 | −26.9 |
| Toxin coregulated pilus biosynthesis protein, TcpF | VC0837 | −45.0 |
| TCP pilus virulence regulatory protein, ToxT | VC0838 | −2.7 |
| Leader peptidase, TcpJ | VC0839 | −18.0 |
| Accessory colonization factor, AcfB | VC0840 | −19.7 |
| Accessory colonization factor, AcfC | VC0841 | −16.3 |
| TagE protein | VC0843 | −10.5 |
| Accessory colonization factor, AcfA | VC0844 | −28.4 |
| Cholera enterotoxin, B subunit, CtxB | VC1456 | −40.4 |
| Cholera enterotoxin, A subunit, CtxA | VC1457 | −39.5 |
| Haemolysin, HlyA | VCA0219 | −6.9 |
| Membrane proteins or secretion | ||
| Transporter, NadC family | VCA0025 | −6.3 |
| Outer membrane protein, OmpV | VC1318 | −4.5 |
| Iron(III) ABC transporter | VCA0687 | −3.6 |
| Amino acid ABC transporter | VC1362 | 4.9 |
| Porin, OmpT | VC1854 | 5.5 |
| Metabolism | ||
| Aldehyde dehydrogenase, AldA-1 | VC0819 | −12.7 |
| Glycerol kinase, GplK | VCA0744 | −5.3 |
| Hydrolase | VCA0877 | 6.9 |
| Acetyl-CoA acetyltransferase | VCA0690 | 8.1 |
| Polyhydroxyalkanoic acid synthase, PhaC | VCA0688 | 9.1 |
| Acetoacetyl-CoA reductase | VCA0691 | 9.9 |
| Chemotaxis and motility | ||
| Methyl-accepting chemotaxis protein | VC1248 | 6.3 |
| Purine-binding chemotaxis protein, CheW | VC1402 | 5.5 |
| Methyl-accepting chemotaxis protein | VC1403 | 4.9 |
| Methyl-accepting chemotaxis protein | VCA0031 | 3.4 |
| Chemotactic transducer-related protein | VCA0895 | 4.0 |
| Methyl-accepting chemotaxis protein | VCA0979 | 4.2 |
| Methyl-accepting chemotaxis protein | VCA1092 | 4.8 |
| Chemotaxis protein, CheA | VCA1095 | 3.8 |
| Chemotaxis protein, CheY | VCA1096 | 3.7 |
| Regulation | ||
| Sensory box sensor histidine kinase | VCA0211 | 3.6 |
| Hemagglutinin/protease regulatory protein, HapR | VC0583 | 6.9 |
| Unknown functions | ||
| Enterobactin synthetase | VC1579 | −9.9 |
| Hcp protein | VC1415 | 4.8 |
| Hcp protein | VCA0017 | 7.1 |
Positive values represent activation in luxO mutants, negative values represent repression. Expression of 20 other conserved hypothetical genes and hypothetical genes is changed greater than 3-fold in luxO mutants (data not shown).