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Restriction mapping of recombinant RevTRE-p53 and RevTet-Off plasmids. The pRevTRE-p53 plasmid was cleaved with Hind III and Cla I. The fragments were as follows: Lane 1: 1 Kb marker, Lane 2: the fragments of the pRevTRE vector (6.5 Kb) and the p53 fragment (2.1 Kb). Lane 3: circled pRevTet-Off (7.8 Kb); Lane 4: circled pRevTRE-p53 (8.6 Kb).
