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Selection of the optimal clone under Dox control. For each of the hygromycin-resistant clones, cells were grown for 24 h in the presence (Dox+) or absence (Dox-) of 1 μg/ml Dox. The expression of p53 protein was analyzed by Western blotting. Lanes 1, 2: 13T (Dox+, Dox-); Lanes 3, 4: 15T (Dox+, Dox-); Lanes 5, 6: 23T (Dox+, Dox-).
