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Semi-quantitative RT-PCR analysis of expression of p53 regulated by Dox. Hep3B/tet/p53-13 (p53-13) cells were cultured for 24 h in the presence or absence of 1 μg/ml Dox and analyzed by semi-quantitative RT-PCR. Lane 1:100 bp marker; Lane 2: Hep3B cells; Lane 3: p53-13 + 1 μg/ml Dox; lane 4: p53-13 + 0 μg/ml Dox. β-microglobulin (MG) was used as an internal control.
