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Expression of p53 protein by Dox controlled quantitatively. After p53-13 cells were cultured in 0 and 1, 0.1, 0.01, 0.001, 0.0001, 0.00001 μg/ml Dox for 24 h, expression of p53 protein was examined by Western blotting. β-actin was served as an internal control.
