Figure 3.

A) Product formed by soluble (black symbols) or inclusion body (red symbols) VP1LAC through ONPG hydrolysis as determined at 414 nm. Very coincident results have been obtained by using CPRG as alternative substrate (see the small panel), whose hydrolysis product was determined at 540 nm. B) Conversion of NADPH into NADP⁺ associated to tetrahydrofolate formation mediated by soluble (black symbols, left scale) and inclusion body (red symbols, right scale) hDHFR. Absorbance was determined at 340 nm.