Figure 4.

The antiviral activity of MxA correlates with the formation of MxA/N complexes. Control cells (a–c) or Vero cells constitutively expressing MxA (d–f) or MxA(E645R) (g–i) were used for infection. In parallel, Vero cells that transiently expressed MxA(L612K) (j–l), MxA(T103A) (m–o), or TMxA (p–r) were used. All cells were infected with 10 pfu/cell of LACV. Twenty hours later, the cells were fixed and analyzed for the viral N (red) and the various MxA proteins (green) by immunofluorescence. Panels at the right show the superimposition of the two images. Bar, 20 μm.