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Amplification, melting curve, and agarose gel electrophoresis of RT-PCR targeting a 220-bp product on ISAV segment 8 using total RNA from ISAV-infected normal CHSE-214 cells: NBISA01 (A-C) and HKS-36 (D-F). Total RNA was subjected to real-time RT-PCR with 50 cycle amplification. The arrows in C and F indicate the expected band of 220 bp on agarose gel.
