Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Amplification, melting curve, and agarose gel electrophoresis of RT-PCR targeting a 220-bp product on ISAV segment 8 using total RNA from ISAV-infected normal CHSE-214 cells constitutively expressing ASMx1: NBISA01 (G-I) and HKS-36 (J-L). Total RNA was subjected to real-time RT-PCR with 50 cycle amplification. The arrows in I and L indicate the expected band of 220 bp on agarose gel.
