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. 2002 Feb 26;99(5):3288–3293. doi: 10.1073/pnas.052693999

Figure 1.

Figure 1

HPLC purification and MS analysis of active fraction from ether extract of FCS. (A) HPLC-UV (250 nm) chromatogram of the second purification step and the protective activity of fractions 10–14. For measurements of protective activity, FCS was extracted with ether and then subjected to the two-step purification. The residue from each fraction, whose amount corresponded to that derived from 300 ml of FCS, was dissolved in 2.5 ml of culture medium and added to the culture for 1 h with SNOC (300 μM). C, control. Values are expressed as the mean ± SE. **, P < 0.01, compared with SNOC alone (Dunnett's two-tailed test). (B) HPLC-MS (m/z 383) chromatogram of the second purification step.