Figure 1.
Activase expression in leaves of the wild type (Wt), rca mutant (rca), and rca transgenics expressing the 43-kDa (43) or 46-kDa (46) isoforms. A typical result is shown in which each lane contained 2 μg of total soluble protein. The figure is a composite created by eliminating intervening lanes on the original immunoblot and the addition of the “rca” lane from a different immunoblot obtained under identical conditions. Immunoblot quantitation by densitometry with recombinant standards indicated that expression in the wild type was 110 mg⋅m−2 at nearly a 1:1 ratio (51% vs. 49%). Expression as compared with 100% for the wild type averaged 71% ± 12% in the 43-kDa plants and 51% ± 8% in the 46-kDa plants (n ≥ 4).