Fig. 3.
Coexpression of C309-driven marking and FRUM protein. Double labeling of CNS tissue dissected from C309/UAS-egfp male adults. Green denotes GFP-expressing cells; magenta denotes anti-FRUM-mediated staining; white denotes coexpressions of the two kinds of signals. These images resulted from scanning 1- to 2-μm sections, then projecting them into a Z-series. (A) Anterior view of adult male (magnification, ×46), showing white colocalization of C309- and fru-expressing cells in the brain (whose total width, between the two distal-most bilaterally symmetrical optic lobes, is ≈450 μm); cells within FRUM clusters 2, 5, 7, and 8 (see Table 2) are represented. (B) Neurons within cluster 2 at magnification ×3.9 that of A. (C) Clusters 5 and 7, magnification ×2 that of A. (D) Ventral nerve cord (VNC), magnification ×1.5 that of A: ventral view of the thoracic ganglia; pro-thoracic region is in the upper right; the mesothoracic region is in the center; part of the metathoracic region is near the bottom left. (E) Abdominal ganglion (posterior-most VNC region), magnification ×1.5 of that in A.