Table 2. FRUM-containing neuronal groups overlapping with expression of the C309 enhancer trap.
| Location | FRUM neuronal group, cluster number | Cluster name | Description of CNS region (numbers of adult FRUM neurons per hemi-brain or—VNC) | FRUM cells overlapping with C309, % (n) | traF-affected FRUM cells overlapping with C309, % (n) | fruMIR-affected FRUM cells overlapping with C309 at 25°C/29°C, % (n) |
|---|---|---|---|---|---|---|
| Brain | 2 | fru-aSP2 | Anterior portion of superior protocerebrum (50 ± 2, n = 7) | 25 (7) | 2 (6) | 15 (9)/10 (5) |
| 5 | fru-mAL | Medially located, just above antennal lobe (34 ± 1, n = 9) | 21 (7) | 2 (6) | 11 (9)/6 (8) | |
| 7 | fru-mcAL | Anterior to mechanosensory neuropile of antennal lobe, near esophagus (36 ± 1, n = 12) | 26 (9) | 2 (7) | 24 (8)/16 (8) | |
| 8 | fru-SG | Within subesophageal ganglion (10 ± 1, n = 6) | 23 (5) | 3 (7) | 24 (3)/18 (4) | |
| 13 | fru-pSP2 | Posterior portion of superior protocerebrum, near mushroom-body (MB) calyx (20 ± 1, n = 5) | 47 (5) | 7 (6) | 52 (5)/30 (5) | |
| 14 | fru-P | Broadly scattered in posterior brain, ventral to MB calyx (78 ± 3, n = 5) | 31 (5) | 3 (7) | 29 (5)/28 (4) | |
| VNC | 16 | fru-Pr | Prothoracic ganglion (29 ± 1, n = 7) | 14 (13) | 4 (6) | 11 (4)/9 (7) |
| 17 | fru-PrMs | Between pro- and mesothoracic ganglion (83 ± 2, n = 8) | 31 (9) | 4 (6) | 35 (7)/29 (6) | |
| 18 | fru-MsMt | Between meso- and metathoracic ganglion (37 ± 1, n = 6) | 30 (5) | 7 (4) | 28 (6)/28 (4) | |
| 20 | fru-Ab | Within abdominal ganglion (99 ± 5, n = 4) | 23 (5) | 3 (7) | 16 (8)/16 (4) |
The second and third columns list FRUM cluster numbers, each followed by the name of a given such neuronal group (compare ref. 16) within the brain or ventral nerve cord (VNC). The fourth column includes novel counts of adult neurons, determined by application of anti-FRUM to CNSs of wild-type (WT) males (average numbers of immunoreactive cells per hemi-CNS region, ± SEM; n, number of specimens scored). An additional series of C309/UAS-traF CNS specimens had anti-FRUM applied for single-labeling; numbers of immunoreactive neurons for the 10 clusters led to an average of 62%, comparing each traF-affected count to the corresponding WT male value (the smaller percentages yielding the 62% average ranged from 42 to 86, n values for these C309/UAS-traF specimens ranged from 6 to 9). Right three columns: percentages for C309/fru coexpression, computed as described in Materials and Methods. Control percentage overlaps (per CNS specimen, whose n values are in parentheses), fifth column. Experimental transgenic coexpression percentages: XY/C309/UAS-egfp/UAS-traF, sixth column; C309/UAS-egfp/UAS-fruMIR, seventh column, based on data collected separately from males reared at 25°C or 29°C. A one-way ANOVA on (on arcsine-transformed percentages) and subsequent pairwise comparisons revealed significant traF-induced overlap reductions (compared with C309/egfp values) for each cluster (P values < 0.05); for C309/fruMIR at 25°C, coexpression reductions were significant only for clusters 2, 5, and 20; at 29°C, fruMIR caused significantly lower coexpression for clusters 2, 5, 7, 13, and 16.