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. 2002 Mar 12;99(6):3412–3416. doi: 10.1073/pnas.042700599

Figure 2.

Figure 2

Incorporation of radiolabeled cocaine into HSA. SDS/PAGE and autoradiogram of (i) HSA exposed to 4′-[125I]iodococaine for 15 min (lane 1, COC 15m) or 3 days (lane 2, COC 3d), 4′-[125I]iodobenzoyl ecgonine for 3 days (lane 3, BE 3d), or 4-[125I]iodobenzoic acid for 3 days (lane 4, BA 3d) or (ii) 4′-[125I]iodococaine incubated for 3 days with HSA methylated with CH2O/NaBH4 (lane 5, Me HSA+COC3d) or N-terminally blocked with glyoxalic acid (lane 6, N-ter HSA COC 3d) or blocked at serine and tyrosine hydroxyl groups with diisopropyl fluorophosphate (lane 7, O-P HSA COC 3d). n = 3; illustrative results are shown.