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. 2002 Mar 12;99(6):3412–3416. doi: 10.1073/pnas.042700599

Figure 4.

Figure 4

Incorporation of anti-benzoyl ecgonine immunoreactivity into proteins. SDS/PAGE chromatography and Western blot with mAb 46H1 of samples are shown. n = 3; illustrative results are shown. (A) HSA (lane 1); HSA incubated with cocaine and immunoprecipitated with mAb 46H1 (lane 2, HSA+COC); and HSA acylated as per Fig. 1C with N-hydroxyphthalimide-activated benzoyl ecgonine (lane 3, BE HSA). (B) Plasma from rats exposed to cocaine twice daily for 7 days (lanes 1–6) and plasma from an unexposed control (lane 7) immunoprecipitated with mAb 46H1. (C) Plasma from human cocaine users (lanes 1–6) and an unexposed control subject (lane 7) immunoprecipitated with mAb 46H1. Each lane represents material from an individual.