Figure 4.

The 3′ end of the rbsK gene directs SsrA tagging in a different context. (A) The nucleotide sequences and corresponding amino acid sequence for the 3′ end of yjgR, the wild-type rpiB gene, and an rpiB mutant containing the 3′ end of the rbsK gene. Asterisks (*) indicate the position of mutated nucleotides relative to wild-type rpiB. (B) Total urea-soluble protein from IPTG-induced and uninduced cells carrying these constructs was resolved by SDS/PAGE, followed by staining with Coomassie blue. The migration position of RpiB is indicated. YjgR has a predicted M_r of 54 kDa. (C) Anti-His6 Western-blot analysis of SsrA(ANDH₆D)-tagged YjgR and SsrA(ANDH₆D)-tagged RpiB.