. 2025 Apr 19;15(6):3163–3180. doi: 10.1016/j.apsb.2025.04.014

Table 2.

Melting temperature of PA-C and variants in the presence and absence of compound 24. Standard deviations were calculated from at least n = 4.

Protein/ligand	Melting temperature (T_m), ^oC, mean ± SD	Change in melting temperature (ΔT_m)), ^oC
PA-C wild type/DMSO control	38.4 ± 0.3	Peak 1: +17.5 Peak 2: +22.5
PA-C wild type/24	Peak 1: 55.9 ± 1.7 Peak 2: 60.9 ± 1.7	Peak 1: +17.5 Peak 2: +22.5
PA-C K643A/DMSO control	50.8 ± 0.8	‒0.6
PA-C K643A/24	50.2 ± 1.9	‒0.6
PA-C Q408A/DMSO control	42.8 ± 0.13	Peak 1: +8.7 Peak 2: +13.1
PA-C Q408A/24	Peak 1: 51.5 ± 1.6 Peak 2: 55.9 ± 0.6	Peak 1: +8.7 Peak 2: +13.1