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Time courses of RNase protection by T. thermophilus EF-Tu⋅GTP for Val-tRNAGly (●), Val-tRNAPro (○), Val-tRNAArg (▴), and Val-tRNAGln (▵) in 0.5M NH4Cl buffer at 2°C. Lines correspond to koff = 0.018, 0.20, 0.33, and 1.6 min−1, respectively.
