Table 1.
Summary of glutathionylated proteins identified
| Protein | NCBI number | Mol mass, kDa | Spot no. (Fig. 2) | Exp. where identified by sequencing | Labeling (summary)
|
||
|---|---|---|---|---|---|---|---|
| C | D | H | |||||
| Stress proteins | |||||||
| HSP70-9B (mortalin-2) | 4758570 | 70 | 1, 2 | 3 | + | + | |
| Stress-induced phosphoprotein 1 | 400042 | 63 | 1 | + | |||
| HSP60 | 4504521 | 60 | 3, 4 | 3 | +/− | ||
| Enzymes | |||||||
| Inosine 5′-monophosphate dehydrogenase 2 | 124419 | 55 | 5 | 3 | +/− | ||
| Protein disulfide isomerase* | 1085373 | 48 | 2 | + | |||
| Enolase 1, alpha | 4503571 | 47 | 8 | 1, 2, 3 | ++/− | † | |
| (C term of) phosphoglycerate kinase | 6456828 | 12, 25 | 2‡, 3 | + | |||
| Aldolase | 229674 | 39 | 14, 17, 18 | 2§, 3 | + | ||
| 6-Phosphogluconolactonase | 6912586 | 27 | 22, 23 | 1, 3 | + | ||
| Phosphorylase kinase delta | 13544110 | 16 | 41 | 3 | + | ||
| Triosephosphate isomerase | 136060 | 26 | 1¶, 2 | + | |||
| Adenylate kinase 2 | 7434595 | 26 | 1 | ||||
| Peroxiredoxin 1 | 4505591 | 22 | 30 | 3 | + | ||
| dUTP pyrophosphatase | 1071889 | 15 | 33 | 3 | + | ||
| Nudix-type motif 6 | 11095443 | 17 | 35 | 3‖ | ++ | + | |
| Peptidylprolyl isomerase (cyclophilin A) | 10863927 | 18 | 40 | 1.3 | − | − | |
| Peptidylprolyl isomerase (cyclophilin A) | 10863927 | 18 | 36–39 | 1, 3 | + | + | |
| Cytochrome c oxidase | 4758038 | 16 | 48 | 3 | + | ||
| Ubiquitin-conjugating enzyme E2N | 4507793 | 17 | 42 | 3 | + | +/− | +/− |
| Cytoskeletal proteins | |||||||
| Vimentin | 13629179 | 53 | 6, 7 | 1, 3 | + | ||
| Vimentin (fragment?) | 13629179 | 53 | 10, 11 | 3 | + | ||
| Actin | 13278939 | 41 | 13 | 1**, 2‡‡, 3 | ++ | ++ | |
| Tropomyosin | 37424 | 29 | 19, 20 | 1, 3 | + | ||
| Transgelin, SM22 homolog calponin-like | 4507357 | 22 | 29 | 1, 2, 3 | ++ | + | |
| Cofilin | 5031635 | 18 | 34 | 1††, 3 | ++ | + | |
| Myosin | 189017 | 11 | 43 | 3 | + | ||
| Profilin | 4826898 | 15 | 45 | 3 | + | ||
| Profilin | 4826898 | 15 | 46 | 1, 2§§, 3 | + | ||
| Other | |||||||
| T complex protein 1 | 6094436 | 58 | 1¶¶ | + | |||
| Lymphocyte-specific protein 1 | 2135579 | 36 | 9 | 3 | + | ||
| Nucleophosmin (dimer?) | 13277532 | 23 | 15 | 1, 2, 3 | + | ||
| Nucleophosmin (fragment?) | 13277532 | 23 | 32 | 3 | + | ||
| Hepatoma-derived growth factor | 4758516 | 27 | 16 | 3 | + | ||
| Translation elongation factor | 4503477 | 25 | 21 | 3 | + | ||
| Endoplasmic reticulum protein | 5803013 | 29 | 23, 24 | 3 | + | ||
| SFR1 splicing factor | 730773 | 28 | 1 | + | |||
| Ash protein | 28876 | 18 | 26 | 3 | + | ||
| RNA-binding protein regulatory subunit | 6005749 | 20 | 27, 28 | 1, 3 | ++ | + | |
| My032 protein | 12002006 | 15 | 31 | 3 | ++ | ||
| Fatty acid binding protein (psoriasis assoc.) | 4557581 | 15 | 44 | 1, 2, 3 | +? | + | |
| Beta galactoside soluble lectin (galectin) | 227920 | 14 | 47∥∥ | 2, 3 | ++ | ||
| 40S ribosomal protein S12 | 133742 | 14 | 1 | +? | + | ||
NCBI, National Center for Biotechnology Information.
Weak identification.
Enolase labelling was different in the three experiments (very high in the first, null in the third).
Only in the place of spot 25 in experiment 3.
Also two low molecular weight fragments.
In the place of phosphogluconolactonase and endoplasmic reticulum protein in experiment 3 (spot 24).
In the place of cofilin in experiment 1.
One spot in the same position as experiment 3, one additional faint spot, more acidic, same molecular mass.
In a different position than experiment 3 (smaller, more basic), probably a fragment.
In a different position from experiment 3 (more basic, same molecular mass).
In a different position from experiment 3 (smaller and more acidic).
In the place of inosine 5′-monophosphate dehydrogenase 2 in experiment 3 (spot 5).
Galectin is also contained in spots a, b, and c.