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Trapping analysis of recombinant NEH1 in extracts of mutM nei E. coli. Lane 1, no protein; lanes 2–4, trapping assay with lysates (1 μg) of E. coli containing empty vector, WT NEH1, and Pro 1 deletion mutant, after incubation with 0.1 pmol of DHU⋅A-containing duplex oligo; lane 5, trapped complex of purified Nth (5 ng) used as a reference with 0.1 pmol of DHU⋅A. The positions of trapped complexes and free DNA are indicated.
