Figure 4.

Restored Fhit expression induces caspase-8 activation. (a) H460 cells and its stable Fhit transfectants clone 2.I and 2.3 were kept in normal culture conditions (10% FCS, +) or serum starved (−) and then analyzed by Western blot by using a caspase-8-specific monoclonal antibody. Presence of cleavage forms of pro-caspase (p43/p41 and the active p18 subunit) indicates activation. (b) Indicated cell lines were infected with adenoviral vectors expressing Fhit (F), lacZ (L), or mock-infected (M) and analyzed after 5 days.