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Analyses of TMV and TMV-T42W RNA accumulation in BY-2 protoplasts. BY-2 protoplasts were inoculated with in vitro transcribed viral RNA, and samples were harvested from time 0 (time of initial contact of RNA with protoplasts) to 50 h after inoculation. Total RNA was purified and 2 μg was analyzed by Northern blot. The position of genomic RNA and subgenomic (sg) RNAs are indicated: sg-RNA I, sg-RNA II (MP subgenomic RNA), and sg-RNAIII (CP subgenomic RNA).
