Table 1.
Mouse ID | Age (months) | Mammary glands
|
Mammary tumors
|
||||
---|---|---|---|---|---|---|---|
Total pfu × 103 | Mutant plaques | Mutant frequency × 10−5 | Total pfu × 103 | Mutant plaques | Mutant frequency × 10−5 | ||
neu-1 | 19 | 381 | 56 | 14.7 | 280 | 98 | 35.0 |
neu-2 | 19 | 274 | 42 | 15.3 | 188 | 72 | 38.3 |
neu-3 | 20 | 220 | 60 | 27.3 | 288 | 123 | 42.7 |
neu-4 | 20 | 263 | 63 | 24.0 | |||
neu-5 | 23 | 272 | 54 | 19.8 | 285 | 98 | 34.4 |
neu-6 | 23 | 178 | 82 | 46.1 | |||
neu-7 | 17 | 184 | 25 | 13.6 | |||
neu-8 | 23 | 233 | 50 | 21.7 | 164 | 180 | 31.7 |
neu-9 | 24 | 223 | 38 | 17.0 | 192 | 56 | 29.2 |
neu-10 | 25 | 234 | 42 | 18.0 | |||
neu-11 | 25 | 102 | 21 | 20.7 | 443 | 64 | 14.4 |
Mean ± SEM | 19.5 ± 2.1 | 29.8 ± 3.3 | |||||
wt-1 | 6.0 | 144 | 21 | 14.6 | |||
wt-2 | 4.5 | 523 | 98 | 18.7 | |||
wt-3 | 4.0 | 220 | 29 | 13.2 | |||
wt-4 | 4.0 | 243 | 26 | 10.7 | |||
wt-5 | 5.5 | 157 | 17 | 10.8 | |||
wt-6 | 4.0 | 148 | 19 | 12.8 | |||
wt-7 | 5.5 | 142 | 18 | 12.7 | |||
Mean ± SEM | 13.4 ± 1.0 |
Tissues are derived from: (neu-1 through neu-11) ErbB2/Big Blue bitransgenic mice carrying both the MMTV-c-erbB2 transgene and a second transgene consisting of multiple copies of the λ bacteriophage genome; or (wt-1 through wt-7) phenotypically wild-type Big Blue single transgenic mice crossed once to FVB/N mice. Note that although the wild-type group was substantially younger than the bitransgenic group, contralateral “normal” mammary tissues from seven tumor-bearing ErbB2/Big Blue bitransgenic mice were subjected to cII mutation analyses and their collective mutant frequency was significantly lower than the frank tumors (P < 0.05).