Fig 1.

 Mouse bone marrow–derived dendritic cells (BMDCs) matured by glycoprotein 96 (Gp96) induce peptide-specific proliferation and interferon (IFN)-γ production by CD8 T cells in vitro. (A) Cytokine production by mouse BMDCs matured by Gp96 or control stimuli for 3 days. (B) Proliferation of St42 CD8 T cells in vitro. BMDCs from C57BL/6 mice were treated with Gp96 or lipopolysaccharides for 3 days and loaded with Ad5-E1A peptide to be used for coculture with carboxyfluorescein diacetate succinimide ester–labeled spleen cells from St42 mice. Proliferation of CD8 T cells was assayed by flow cytometric analysis on day 4 after T-cell stimulation. (C) IFN-γ production of St42 CD8 T cells in vitro. The graph shows the percentage of intracellular IFN-γ production of the most proliferated CD8 T cells on day 5 (see insert). (D) Interleukin-12 production of the BMDCs used for the in vitro activation of St42 T cells. The results are representative of 3 independent experiments