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. 2001 Jun 1;69(1):35–48. doi: 10.1086/321265

Figure 3.

Figure 3

Increase in the PEX1 protein level of patient fibroblasts harboring the G843D allele, at 30°C. Cells were cultured for 3 d at 37°C or 30°C. Cell lysates were subjected to SDS-PAGE and transferred onto nitrocellulose. Immunoblot analysis was performed, using polyclonal antibodies against PEX1 protein. WT = wild type (GM5659); “31” and “27” refer to patients 31 and 27, respectively. An arrow (←) marks the position of the PEX1 protein; the asterisk (*) denotes an unspecific protein recognized by anti-PEX1 antibodies, which was used to correct for an eventually unequal loading of the sample. The protein levels are shown in table 4. A, Comparison of wild-type (GM5659) cells versus cells from patients 31 and 27, at 30°C and 37°C. In comparison with wild-type cells, we detected ∼5%–17% of the normal level of PEX1 protein in lysates of cells incubated at 37°C, and ∼17%–28% of the normal level of PEX1 protein in those incubated at 30°C (see table 4 for protein levels). This increase in protein levels may be explained by an improved stability of PEX1 at the lower temperature. B, Lower PEX1 protein level in control wild-type fibroblasts at 30°C compared with that at 37°C, under the same conditions as above.