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. 2001 Mar 29;68(5):1102–1109. doi: 10.1086/320125

Figure 4.

Figure 4

Regulation of Dax1 by Wnt-4 and SF1. In a, the upper panel shows expression of Dax1 in Sertoli (TM4) cells, by RT-PCR after transfection with Wnt-4 (lanes 1–3), Wnt-4 and SF1 (lanes 4–6), SF1 (lanes 7–9), and Renilla luciferase control vector (lane 10), and the lower panel shows expression of β-actin from the same samples. In b, the upper panel shows expression of Dax1 in Leydig (MLTC-1) cells, by RT-PCR after transfection with Wnt-4 (lanes 1 and 2), Wnt-4 and SF1 (lanes 3 and 4), and Renilla luciferase control vector (lane 5), and the lower panel shows expression of β-actin from the same samples. The templates for positive and negative controls are mouse ovarian RNA and water, respectively. In c, the upper panel shows expression of Dax1 in Sertoli (TM4) cells, by northern blot after transfection with Wnt-4 (lanes 1–3), Wnt-4 and SF1 (lanes 4–6), SF1 (lanes 7–9), and pcDNA3.1 (lanes 10–12), as well as RNA from a mock-transfected control (lane 13), and the lower panel shows expression of β-actin on the same blot.