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. 2001 Apr 26;68(6):1398–1407. doi: 10.1086/320594

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© 2001 by The American Society of Human Genetics. All rights reserved.

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Expression of genes from active or inactive X-containing hybrids. cDNA from mouse/human somatic-cell hybrids that retained either the human active (Xa) or inactive (Xi) X chromosome was amplified with gene-specific primers. A negative image of ethidium bromide–stained products separated by agarose gel electrophoresis is shown. Lanes contain human female cell line (lane 1); mouse tsA1S9az31b cell line (lane 2); t60-12 (lane 3); Aha11aB1 (lane 4); t86-B1maz1b-3a (lane 5); t11-4Aaz5 (lane 6); t75-2maz34-4a (lane 7); t48-1a-Daz4a (lane 8); LT23-1E2Buv5Cl26-7A2 (lane 9); L23-4B (lane 10); t51-S2 (lane 11); tHM-1a (lane 12); tHM-1d (lane 13); A23-1aCl5 (lane 14); genomic DNA from a somatic-cell hybrid cell line retaining human chromosome 19 (lane 15). PCR products in all lanes have been digested with BsaJ1 to distinguish SEDL product from pseudogene SEDLP product, since only the latter is cleaved by BsaJ1. Control transcripts A007K03 (which is subject to inactivation) and MIC2 (which escapes inactivation) demonstrate the inactivation status for each hybrid shown.