Figure 2.

CD95-mediated apoptosis of CD40-activated CLL cells. CLL cells were CD40-activated and harvested 1, 3, or 5 days later for use as targets in a DiOC₆ apoptosis assay. CHO (open bars), CHO-CD95-L (black bars), or anti-CD95L mAb pretreated CHO-CD95-L (hatched bars) effector cells were cocultured with target cells at an effector-to-target cell ratio of 10:1 for 8 h at 37°C. DiOC₆ dull or apoptotic target cells were measured by flow cytometric analysis. Data represent the mean ± SD from experiments testing seven different CLL patient samples.