Figure 5.

Down-regulation of FLIP in CLL cells. (A) CLL cells were electroporated with PBS, FLIP antisense, or control scrambled oligo as described. Cells were then CD40 activated for 24 h and harvested for FLIP immunoblot analysis, or (B) CD95 apoptosis assays, as described. Bars indicate the % apoptosis of control-treated CLL cells (open bars), FLIP-antisense-treated CLL cells (black bars), or JURKAT cells (hatched bars) when incubated with CHO cells or CHO cells that express CD95-L (CHO-CD95-L).